Coding

Part:BBa_K2788000:Design

Designed by: Lindong He   Group: iGEM18_SZU-China   (2018-10-05)


HsbA_Beauveria bassiana ARSEF 2860.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 684
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 244
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The studies on this protein are slim, so there is no prepared antibody on sale. If you want to study on its expression, for example, you want to do Western Blot and other technique based on antigen-antibody reaction, you'd better customize antibody in advance. Besides, preparation of his-tag fusion protein is an alternative proposal.


Source

/organism="Beauveria bassiana ARSEF 2860"
/strain="ARSEF 2860"
/culture_collection="ARSEF:2860"
/db_xref="taxon:655819"
/chromosome="Unknown"

It's from Beauveria bassiana ARSEF 2860

References

[1]Xiao G, Ying S H, Peng Z, et al. Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana[J]. Scientific Reports, 2012, 2(483):483.

[2]Ye Zhang, Zhongren Lei, Haihong Wang, Jiqing Zhan. Prokaryotic expression and immunolocalization Beauveria bassiana HsbA protein [J] Chinese Agricultural Sciences, 2013,46 (21): 4534-4541.